WEKO3
アイテム
{"_buckets": {"deposit": "88be6dbd-3f35-44fd-8be6-e800a6888f58"}, "_deposit": {"id": "46428", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "46428"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00046428", "sets": ["2600", "2793", "6608"]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "136", "bibliographicPageStart": "125", "bibliographicVolumeNumber": "8", "bibliographic_titles": [{"bibliographic_title": "International Journal for Parasitology: Drugs and Drug Resistance"}]}]}, "item_5_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "橋本, 哲男"}], "nameIdentifiers": [{"nameIdentifier": "130586", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "50208451", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000050208451"}, {"nameIdentifier": "0000001162", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001162"}]}, {"creatorNames": [{"creatorName": "松田, 真秀"}], "nameIdentifiers": [{"nameIdentifier": "204852", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "30614333", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000030614333"}, {"nameIdentifier": "0000003630", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000003630"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The Coenzyme A (CoA), as a cofactor involved in \u003e100 metabolic reactions, is essential to the basic biochemistry of life. Here, we investigated the CoA biosynthetic pathway of Entamoeba histolytica (E. histolytica), an enteric protozoan parasite responsible for human amebiasis. We identified four key enzymes involved in the CoA pathway: pantothenate kinase (PanK, EC 2.7.1.33), bifunctional phosphopantothenate-cysteine ligase/decarboxylase (PPCS-PPCDC), phosphopantetheine adenylyltransferase (PPAT) and dephospho-CoA kinase (DPCK). Cytosolic enzyme PanK, was selected for further biochemical, genetic, and phylogenetic characterization. Since E. histolytica PanK (EhPanK) is physiologically important and sufficiently divergent from its human orthologs, this enzyme represents an attractive target for the development of novel anti-amebic chemotherapies. Epigenetic gene silencing of PanK resulted in a significant reduction of PanK activity, intracellular CoA concentrations, and growth retardation in vitro, reinforcing the importance of this gene in E. histolytica. Furthermore, we screened the Kitasato Natural Products Library for inhibitors of recombinant EhPanK, and identified 14 such compounds. One compound demonstrated moderate inhibition of PanK activity and cell growth at a low concentration, as well as differential toxicity towards E. histolytica and human cells.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier "}]}, "item_5_relation_10": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "29518650", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.ijpddr.2018.02.004", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2018 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/)"}]}, "item_5_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "22113207", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nurkanto, Arif"}], "nameIdentifiers": [{"nameIdentifier": "189319", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Jeelani, Ghulam"}], "nameIdentifiers": [{"nameIdentifier": "189320", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamoto, Takehiro"}], "nameIdentifiers": [{"nameIdentifier": "189321", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Naito, Yoshiko"}], "nameIdentifiers": [{"nameIdentifier": "189322", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hishiki, Takako"}], "nameIdentifiers": [{"nameIdentifier": "189323", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mori, Mihoko"}], "nameIdentifiers": [{"nameIdentifier": "189324", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suematsu, Makoto"}], "nameIdentifiers": [{"nameIdentifier": "189325", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shiomi, Kazuro"}], "nameIdentifiers": [{"nameIdentifier": "189326", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hashimoto, Tetsuo"}], "nameIdentifiers": [{"nameIdentifier": "189327", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nozaki, Tomoyoshi"}], "nameIdentifiers": [{"nameIdentifier": "189328", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-05-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "IJP_8-1.pdf", "filesize": [{"value": "1.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_6", "mimetype": "application/pdf", "size": 1200000.0, "url": {"label": "IJP_8-1", "url": "https://tsukuba.repo.nii.ac.jp/record/46428/files/IJP_8-1.pdf"}, "version_id": "3559ac37-e1d2-4141-96d2-3345b4267a95"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Characterization and validation of Entamoeba histolytica pantothenate kinase as a novel anti-amebic drug target", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Characterization and validation of Entamoeba histolytica pantothenate kinase as a novel anti-amebic drug target"}]}, "item_type_id": "5", "owner": "1", "path": ["2600", "2793", "6608"], "permalink_uri": "http://hdl.handle.net/2241/00151709", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-05-21"}, "publish_date": "2018-05-21", "publish_status": "0", "recid": "46428", "relation": {}, "relation_version_is_last": true, "title": ["Characterization and validation of Entamoeba histolytica pantothenate kinase as a novel anti-amebic drug target"], "weko_shared_id": 5}
Characterization and validation of Entamoeba histolytica pantothenate kinase as a novel anti-amebic drug target
http://hdl.handle.net/2241/00151709
http://hdl.handle.net/2241/00151709ec1d432c-cad5-4bae-9489-095c584b672d
名前 / ファイル | ライセンス | アクション |
---|---|---|
IJP_8-1 (1.2 MB)
|
Item type | Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2018-05-21 | |||||
タイトル | ||||||
タイトル | Characterization and validation of Entamoeba histolytica pantothenate kinase as a novel anti-amebic drug target | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Nurkanto, Arif
× Nurkanto, Arif× Jeelani, Ghulam× Yamamoto, Takehiro× Naito, Yoshiko× Hishiki, Takako× Mori, Mihoko× Suematsu, Makoto× Shiomi, Kazuro× Hashimoto, Tetsuo× Nozaki, Tomoyoshi |
|||||
著者別名 |
橋本, 哲男
× 橋本, 哲男× 松田, 真秀 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The Coenzyme A (CoA), as a cofactor involved in >100 metabolic reactions, is essential to the basic biochemistry of life. Here, we investigated the CoA biosynthetic pathway of Entamoeba histolytica (E. histolytica), an enteric protozoan parasite responsible for human amebiasis. We identified four key enzymes involved in the CoA pathway: pantothenate kinase (PanK, EC 2.7.1.33), bifunctional phosphopantothenate-cysteine ligase/decarboxylase (PPCS-PPCDC), phosphopantetheine adenylyltransferase (PPAT) and dephospho-CoA kinase (DPCK). Cytosolic enzyme PanK, was selected for further biochemical, genetic, and phylogenetic characterization. Since E. histolytica PanK (EhPanK) is physiologically important and sufficiently divergent from its human orthologs, this enzyme represents an attractive target for the development of novel anti-amebic chemotherapies. Epigenetic gene silencing of PanK resulted in a significant reduction of PanK activity, intracellular CoA concentrations, and growth retardation in vitro, reinforcing the importance of this gene in E. histolytica. Furthermore, we screened the Kitasato Natural Products Library for inhibitors of recombinant EhPanK, and identified 14 such compounds. One compound demonstrated moderate inhibition of PanK activity and cell growth at a low concentration, as well as differential toxicity towards E. histolytica and human cells. | |||||
書誌情報 |
International Journal for Parasitology: Drugs and Drug Resistance 巻 8, 号 1, p. 125-136, 発行日 2018-04 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 22113207 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 29518650 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ijpddr.2018.02.004 | |||||
権利 | ||||||
権利情報 | © 2018 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/) | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Elsevier |