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Knock-out transmembrane prostate androgen-induced protein gene suppressed triple-negative breast cancer cell proliferation
http://hdl.handle.net/2241/00149880
http://hdl.handle.net/2241/001498805ac3867e-aaab-41c2-af71-49818015df59
名前 / ファイル | ライセンス | アクション |
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MJI_26-3 (621.1 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2018-01-04 | |||||
タイトル | ||||||
タイトル | Knock-out transmembrane prostate androgen-induced protein gene suppressed triple-negative breast cancer cell proliferation | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Wardhani, Bantari W.K.
× Wardhani, Bantari W.K.× Puteri, Meidi U.× Watanabe, Yukihide× Louisa, Melva× Setiabudy, Rianto× Kato, Mitsuyasu |
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著者別名 |
加藤, 光保
× 加藤, 光保× 渡邊, 幸秀 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Triple negative breast cancer (TNBC) tends to grow more rapidly and has poorer prognosis compared to others. High expression of transmembrane prostate androgen-induced protein (TMEPAI) correlates with poor prognosis in TNBC patients. However, the mechanistic role of TMEPAI in tumorigenic remains unknown. This study aimed to knock-out TMEPAI in TNBC cell line to determine its function further in cells proliferation. Methods: CRISPR-Cas9 has been used previously to knock-out TMEPAI in Hs857T TNBC cell line. Hs587T TNBC parental cell line (wild-type/WT) and TMEPAI knock out Hs 586T cell lines were cultured in Dulbecco’s modified eagle medium (DMEM) supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin and amphotericin B. Both cell lines were seeded in 24-well plates and counted every two days, then proliferation rates were plotted. Afterwards, total RNA were isolated from the cells and Ki-67, and TGF-β mRNA expression levels as proliferation markers were determined. Results: Cell proliferation rates as displayed in growth curve plots showed that WT-TMEPAI cell line grew more rapidly than KO-TMEPAI. In accordance, mRNA expression levels of Ki-67 and TGF-β were significantly decreased KO-TMEPAI as compare to TMEPAI-WT. Conclusion: Knock-out of TMEPAI attenuates cell proliferation in TNBC. |
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書誌情報 |
Medical journal of Indonesia 巻 26, 号 3, p. 178-182, 発行日 2017-09 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2252-8083 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1113872X | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.13181/mji.v26i3.1823 | |||||
権利 | ||||||
権利情報 | @ 2017 Authors | |||||
権利 | ||||||
権利情報 | This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are properly cited. | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | University of Indonesia, Faculty of Medicine |