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Reciprocal complementation of bovine parainfluenza virus type 3 lacking either the membrane or fusion gene
http://hdl.handle.net/2241/00149150
http://hdl.handle.net/2241/00149150ce383e1c-536f-4179-b81d-ea97dd11fb8f
名前 / ファイル | ライセンス | アクション |
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JVM_249-25-30 (466.6 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-11-27 | |||||
タイトル | ||||||
タイトル | Reciprocal complementation of bovine parainfluenza virus type 3 lacking either the membrane or fusion gene | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Takada, Marina
× Takada, Marina× Matsuura, Ryosuke× Kokuho, Takehiro× Tsuboi, Takamitsu× Kameyama, Ken-ichiro× Takeuchi, Kaoru |
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著者別名 |
竹内, 薫
× 竹内, 薫 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Two defective bovine parainfluenza virus type 3 (BPIV3) strains were generated, one lacking the membrane (M) protein gene and expressing EGFP (ΔM-EGFP) and the other lacking the fusion (F) protein gene and expressing mStrawberry (ΔF-mSB), by supplying deficient proteins in trans. When Madin-Darby bovine kidney (MDBK) cells were co-infected with ΔM-EGFP and ΔF-mSB at a multiplicity of infection (MOI) of 0.1, complemented viruses were easily obtained. Complemented viruses grew as efficiently as wild-type BPIV3 and could be passaged in MDBK cell cultures even at an MOI of 0.01, possibly due to multiploid virus particles containing genomes of both ΔM-EGFP and ΔF-mSB. This reciprocal complementation method using two defective viruses would be useful to express large or multiple proteins in cell cultures using paramyxovirus vectors. | |||||
書誌情報 |
Journal of virological methods 巻 249, p. 25-30, 発行日 2017-11 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0166-0934 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00259584 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 28842134 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jviromet.2017.08.010 | |||||
権利 | ||||||
権利情報 | © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Elsevier |