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Phenylpropanoid 2,3-dioxygenase involved in the cleavage of the ferulic acid side chain to form vanillin and glyoxylic acid in Vanilla planifolia
http://hdl.handle.net/2241/00148360
http://hdl.handle.net/2241/00148360b6827d7e-2cb3-4d4a-b27b-280175133175
名前 / ファイル | ライセンス | アクション |
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BBB_81-9 (3.3 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-09-27 | |||||
タイトル | ||||||
タイトル | Phenylpropanoid 2,3-dioxygenase involved in the cleavage of the ferulic acid side chain to form vanillin and glyoxylic acid in Vanilla planifolia | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Negishi, Osamu
× Negishi, Osamu× Negishi, Yukiko |
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著者別名 |
根岸, 紀
× 根岸, 紀 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Enzyme catalyzing the cleavage of the phenylpropanoid side chain was partially purified by ion exchange and gel filtration column chromatography after (NH4)2SO4 precipitation. Enzyme activities were dependent on the concentration of dithiothreitol (DTT) or glutathione (GSH) and activated by addition of 0.5 mM Fe2+. Enzyme activity for ferulic acid was as high as for 4-coumaric acid in the presence of GSH, suggesting that GSH acts as an endogenous reductant in vanillin biosynthesis. Analyses of the enzymatic reaction products with quantitative NMR (qNMR) indicated that an amount of glyoxylic acid (GA) proportional to vanillin was released from ferulic acid by the enzymatic reaction. These results suggest that phenylpropanoid 2,3-dioxygenase is involved in the cleavage of the ferulic acid side chain to form vanillin and GA in Vanilla planifolia. | |||||
書誌情報 |
Bioscience, biotechnology, and biochemistry 巻 81, 号 9, p. 1732-1740, 発行日 2017-07 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0916-8451 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10824164 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 28743207 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1080/09168451.2017.1353402 | |||||
権利 | ||||||
権利情報 | This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, biotechnology, and biochemistry on 26 Jul 2017, available online: http://www.tandfonline.com/10.1080/09168451.2017.1353402. | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Taylor & Francis |