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Evidence that differentiation-inducing factor-1 controls chemotaxis and cell differentiation, at least in part, via mitochondria in D.discoideum
http://hdl.handle.net/2241/00146706
http://hdl.handle.net/2241/0014670629511f57-2750-46c4-80c1-cf2eb86c3394
名前 / ファイル | ライセンス | アクション |
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BO_6-6 (4.0 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-07-04 | |||||
タイトル | ||||||
タイトル | Evidence that differentiation-inducing factor-1 controls chemotaxis and cell differentiation, at least in part, via mitochondria in D.discoideum | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Kubohara, Yuzuru
× Kubohara, Yuzuru× Kikuchi, Haruhisa× Nguyen, Van Hai× Kuwayama, Hidekazu× Oshima, Yoshiteru |
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著者別名 |
桑山, 秀一
× 桑山, 秀一 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Differentiation-inducing factor-1 [1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one (DIF-1)] is an important regulator of cell differentiation and chemotaxis in the development of the cellular slime mold Dictyostelium discoideum. However, the entire signaling pathways downstream of DIF-1 remain to be elucidated. To characterize DIF-1 and its potential receptor(s), we synthesized two fluorescent derivatives of DIF-1, boron-dipyrromethene (BODIPY)-conjugated DIF-1 (DIF-1-BODIPY) and nitrobenzoxadiazole (NBD)-conjugated DIF-1 (DIF-1-NBD), and investigated their biological activities and cellular localization. DIF-1-BODIPY (5 µM) and DIF-1 (2 nM) induced stalk cell differentiation in the DIF-deficient strain HM44 in the presence of cyclic adenosine monosphosphate (cAMP), whereas DIF-1-NBD (5 µM) hardly induced stalk cell differentiation under the same conditions. Microscopic analyses revealed that the biologically active derivative, DIF-1-BODIPY, was incorporated by stalk cells at late stages of differentiation and was localized to mitochondria. The mitochondrial uncouplers carbonyl cyanide m-chlorophenylhydrazone (CCCP), at 25–50 nM, and dinitrophenol (DNP), at 2.5–5 µM, induced partial stalk cell differentiation in HM44 in the presence of cAMP. DIF-1-BODIPY (1–2 µM) and DIF-1 (10 nM), as well as CCCP and DNP, suppressed chemotaxis in the wild-type strain Ax2 in shallow cAMP gradients. These results suggest that DIF-1-BODIPY and DIF-1 induce stalk cell differentiation and modulate chemotaxis, at least in part, by disturbing mitochondrial activity. | |||||
書誌情報 |
Biology open 巻 6, 号 6, p. 741-751, 発行日 2017-06 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2046-6390 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 28619991 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1242/bio.021345 | |||||
権利 | ||||||
権利情報 | © 2017. Published by The Company of Biologists Ltd | |||||
権利 | ||||||
権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Company of Biologists |