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Electrochemical Control of Bioluminescence by Blocking the Adsorption of the Bacterial Luciferase Using a Mercaptobipyridine Self-assembled Monolayer
http://hdl.handle.net/2241/00145999
http://hdl.handle.net/2241/0014599931f7ad34-05a2-46cf-8c06-af2445c9ca70
名前 / ファイル | ライセンス | アクション |
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AS_33-3 (746.6 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-04-27 | |||||
タイトル | ||||||
タイトル | Electrochemical Control of Bioluminescence by Blocking the Adsorption of the Bacterial Luciferase Using a Mercaptobipyridine Self-assembled Monolayer | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
YAMASAKI, Shinya
× YAMASAKI, Shinya× YAMADA, Shuto× TAKEMURA, Hiroyuki× TAKEHARA, Kô |
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著者別名 |
山﨑, 信哉
× 山﨑, 信哉 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | An N-butyl-N′-(4-mercaptobutyl)-4,4′-bipyridinium (4BMBP) was modified on a gold electrode to improve the electrochemical control of the bacterial luciferase (BL) luminescence system. The 4BMBP-modified gold electrode (4BMBP/Au) was able to prevent the adsorption of BL on the electrode surface, and enhanced the electrochemical regeneration rate of the reduced flavin mononucleotide (FMNH2), which is one of the substrates of the BL luminescence reaction. By using the 4BMBP/Au, the luminescence intensity increased by about 27% compared to that of a bare gold electrode (bare Au). Moreover, the modified electrode improved the time required for analysis because the modified layer prevented BL adsorption. Even without a refreshing procedure for each measurement, a constant luminescence intensity could be observed, and the analysis time was reduced to half (about 10 min) for one sample. The 4BMBP/Au is not only useful to control of the BL luminescence system, but also for electrochemical measurements in the presence of proteins. | |||||
書誌情報 |
Analytical sciences 巻 33, 号 3, p. 307-311, 発行日 2017-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0910-6340 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10500785 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 28302971 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2116/analsci.33.307 | |||||
権利 | ||||||
権利情報 | 2017 © The Japan Society for Analytical Chemistry | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | The Japan Society for Analytical Chemistry |