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Crystal structure of an IclR homologue from Microbacterium sp. strain HM58-2
http://hdl.handle.net/2241/00145480
http://hdl.handle.net/2241/00145480451dea86-2b66-4c6d-ba8c-5822a85c24fb
名前 / ファイル | ライセンス | アクション |
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ActaCF_73-1 (1.7 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-03-08 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Crystal structure of an IclR homologue from Microbacterium sp. strain HM58-2 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
Akiyama, Tomonori
× Akiyama, Tomonori× Yamada, Yusuke× 高谷, 直樹× Ito, Shinsaku× Sasaki, Yasuyuki× Yajima, Shunsuke |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The bacterial transcription factor IclR (isocitrate lyase regulator) is a member of a one-component signal transduction system, which shares the common motif of a helix–turn–helix (HTH)-type DNA-binding domain (DBD) connected to a substrate-binding domain (SBD). Here, the crystal structure of an IclR homologue (Mi-IclR) from Microbacterium sp. strain HM58-2, which catabolizes acylhydrazide as the sole carbon source, is reported. Mi-IclR is expected to regulate an operon responsible for acylhydrazide degradation as an initial step. Native single-wavelength anomalous diffraction (SAD) experiments were performed in combination with molecular replacement. CRANK2 from the CCP4 suite successfully phased and modelled the complete structure of a homotetramer composed of 1000 residues in an asymmetric unit, and the model was refined to 2.1 Å resolution. The overall structure of Mi-IclR shared the same domain combination as other known IclR structures, but the relative geometry between the DBD and SBD differs. Accordingly, the geometry of the Mi-IclR tetramer was unique: the putative substrate-binding site in each subunit is accessible from the outside of the tetramer, as opposed to buried inside as in the previously known IclR structures. These differences in the domain geometry may contribute to the transcriptional regulation of IclRs. | |||||
言語 | en | |||||
書誌情報 |
en : Acta Crystallographica Section F Structural Biology Communications 巻 73, 号 1, p. 16-23, 発行日 2017-01 |
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ISSN | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2053-230X | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 28045389 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1107/S2053230X16019208 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | © 2017 International Union of Crystallography | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | International Union of Crystallography |