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NML-mediated rRNA base methylation links ribosomal subunit formation to cell proliferation in a p53-dependent manner
http://hdl.handle.net/2241/00143732
http://hdl.handle.net/2241/0014373217c00081-9137-4af3-bc80-3e41b3335d57
名前 / ファイル | ライセンス | アクション |
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JCS_129-12 (2.7 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2016-08-30 | |||||
タイトル | ||||||
タイトル | NML-mediated rRNA base methylation links ribosomal subunit formation to cell proliferation in a p53-dependent manner | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Waku, Tsuyoshi
× Waku, Tsuyoshi× Nakajima, Yuka× Yokoyama, Wataru× Nomura, Naoto× Kako, Koichiro× Kobayashi, Akira× Shimizu, Toshiyuki× Fukamizu, Akiyoshi |
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著者別名 |
加香, 孝一郎
× 加香, 孝一郎× 深水, 昭吉 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Ribosomal RNAs (rRNAs) act as scaffolds and ribozymes in ribosomes, and these functions are modulated by post-transcriptional modifications. However, the biological role of base methylation, a well-conserved modification of rRNA, is poorly understood. Here, we demonstrate that a nucleolar factor, nucleomethylin (NML; also known as RRP8), is required for the N1-methyladenosine (m1A) modification in 28S rRNAs of human and mouse cells. NML also contributes to 60S ribosomal subunit formation. Intriguingly, NML depletion increases 60S ribosomal protein L11 (RPL11) levels in the ribosome-free fraction and protein levels of p53 through an RPL11–MDM2 complex, which activates the p53 pathway. Consequently, the growth of NML-depleted cells is suppressed in a p53-dependent manner. These observations reveal a new biological function of rRNA base methylation, which links ribosomal subunit formation to p53-dependent inhibition of cell proliferation in mammalian cells. | |||||
書誌情報 |
Journal of cell science 巻 129, 号 12, p. 2382-2393, 発行日 2016-06 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0021-9533 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00694823 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 27149924 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1242/jcs.183723 | |||||
権利 | ||||||
権利情報 | © 2016. Published by The Company of Biologists Ltd | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | The Company of Biologists Ltd |