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Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source
http://hdl.handle.net/2241/00124788
http://hdl.handle.net/2241/0012478802b76e88-58c6-408f-b404-31c8ba946e09
名前 / ファイル | ライセンス | アクション |
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AS_31-4 (2.8 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2015-06-15 | |||||
タイトル | ||||||
タイトル | Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
SEGAWA, Hiroki
× SEGAWA, Hiroki× OKUNO, Masanari× LEPROUX, Philippe× COUDERC, Vincent× OZAWA, Takeaki× KANO, Hideaki |
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著者別名 |
奥野, 将成
× 奥野, 将成× 加納, 英明 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The subnanosecond “white-light laser” source has been applied to multimodal, multiphoton, and multiplex spectroscopic imaging (M3 spectroscopic imaging) with coherent anti-Stokes Raman scattering (CARS), third-order sum frequency generation (TSFG), and two-photon excitation fluorescence (TPEF). As the proof-of-principle experiment, we performed simultaneous imaging of polystyrene beads with TSFG and TPEF. This technique is then applied to live cell imaging. Mouse L929 fibroblastic cells are clearly visualized by CARS, TSFG, and TPEF processes. M3 spectroscopic imaging provides various and unique cellular information with different image contrast based on each multiphoton process. | |||||
書誌情報 |
Analytical sciences 巻 31, 号 4, p. 299-305, 発行日 2015-04 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0910-6340 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10500785 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 25864673 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2116/analsci.31.299 | |||||
権利 | ||||||
権利情報 | 2015 © The Japan Society for Analytical Chemistry | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | The Japan Society for Analytical Chemistry |