WEKO3
アイテム
{"_buckets": {"deposit": "189f26b6-4262-4196-b510-dc23948e0736"}, "_deposit": {"id": "33247", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "33247"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00033247", "sets": ["306", "3017"]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "592", "bibliographicPageStart": "587", "bibliographicVolumeNumber": "104", "bibliographic_titles": [{"bibliographic_title": "Journal of pharmaceutical sciences"}]}]}, "item_5_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "白木, 賢太郎"}], "nameIdentifiers": [{"nameIdentifier": "79", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90334797", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000090334797"}, {"nameIdentifier": "0000000710", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000000710"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Noncovalent PEGylation has great potential for stabilization of therapeutic proteins. Here, we demonstrated that the noncovalent PEGylation with a PEGylated polyelectrolyte stabilized a therapeutic protein, l-asparaginase (ASNase). Anionic ASNase and cationic poly(ethylene glycol)-block-poly(N,N-dimethylaminoethyl methacrylate) (PEG-b-PAMA) formed a water-soluble protein–polyelectrolyte complex (PPC) without loss of secondary structure and enzyme activity. PPC with PEG-b-PAMA successfully inhibited the shaking-induced inactivation and aggregation of ASNase as well as protease digestion, corresponding to the behaviors of covalently PEGylated ASNase. Thus, noncovalent PEGylation by PEGylated polyelectrolytes is a new candidate for handling of therapeutic proteins.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Pharmaceutical Association"}, {"subitem_publisher": "Wiley"}]}, "item_5_relation_10": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "25354692", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1002/jps.24217", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2014 Wiley Periodicals, Inc. and the American Pharmacists Association"}, {"subitem_rights": "This is tthe peer reviewed version of the following article: J. Pharm. Sci., 104: 587–592. doi: 10.1002/jps.24217, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jps.24217/epdf. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."}]}, "item_5_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0022-3549", "subitem_source_identifier_type": "ISSN"}, {"subitem_source_identifier": "1520-6017", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00704450", "subitem_source_identifier_type": "NCID"}]}, "item_5_subject_20": {"attribute_name": "NIIサブジェクト", "attribute_value_mlt": [{"subitem_subject": "基礎工学・応用物理学", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kurinomaru, Takaaki"}], "nameIdentifiers": [{"nameIdentifier": "112828", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shiraki, Kentaro"}], "nameIdentifiers": [{"nameIdentifier": "112829", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-03-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "JPS_104-2.pdf", "filesize": [{"value": "732.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 732200.0, "url": {"label": "JPS_104-2", "url": "https://tsukuba.repo.nii.ac.jp/record/33247/files/JPS_104-2.pdf"}, "version_id": "8ad7a2e6-86b7-40b7-b8f1-c513e2958acf"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Noncovalent PEGylation of l-Asparaginase Using PEGylated Polyelectrolyte", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Noncovalent PEGylation of l-Asparaginase Using PEGylated Polyelectrolyte"}]}, "item_type_id": "5", "owner": "1", "path": ["306", "3017"], "permalink_uri": "http://hdl.handle.net/2241/00124109", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-04-06"}, "publish_date": "2015-04-06", "publish_status": "0", "recid": "33247", "relation": {}, "relation_version_is_last": true, "title": ["Noncovalent PEGylation of l-Asparaginase Using PEGylated Polyelectrolyte"], "weko_shared_id": 5}
Noncovalent PEGylation of l-Asparaginase Using PEGylated Polyelectrolyte
http://hdl.handle.net/2241/00124109
http://hdl.handle.net/2241/001241096249bf64-eaea-4c9e-922c-914d2c1d9dcc
名前 / ファイル | ライセンス | アクション |
---|---|---|
JPS_104-2 (732.2 kB)
|
|
Item type | Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2015-04-06 | |||||
タイトル | ||||||
タイトル | Noncovalent PEGylation of l-Asparaginase Using PEGylated Polyelectrolyte | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Kurinomaru, Takaaki
× Kurinomaru, Takaaki× Shiraki, Kentaro |
|||||
著者別名 |
白木, 賢太郎
× 白木, 賢太郎 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Noncovalent PEGylation has great potential for stabilization of therapeutic proteins. Here, we demonstrated that the noncovalent PEGylation with a PEGylated polyelectrolyte stabilized a therapeutic protein, l-asparaginase (ASNase). Anionic ASNase and cationic poly(ethylene glycol)-block-poly(N,N-dimethylaminoethyl methacrylate) (PEG-b-PAMA) formed a water-soluble protein–polyelectrolyte complex (PPC) without loss of secondary structure and enzyme activity. PPC with PEG-b-PAMA successfully inhibited the shaking-induced inactivation and aggregation of ASNase as well as protease digestion, corresponding to the behaviors of covalently PEGylated ASNase. Thus, noncovalent PEGylation by PEGylated polyelectrolytes is a new candidate for handling of therapeutic proteins. | |||||
書誌情報 |
Journal of pharmaceutical sciences 巻 104, 号 2, p. 587-592, 発行日 2015-02 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0022-3549 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1520-6017 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00704450 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 25354692 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1002/jps.24217 | |||||
権利 | ||||||
権利情報 | © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association | |||||
権利 | ||||||
権利情報 | This is tthe peer reviewed version of the following article: J. Pharm. Sci., 104: 587–592. doi: 10.1002/jps.24217, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jps.24217/epdf. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | American Pharmaceutical Association | |||||
出版者 | ||||||
出版者 | Wiley |