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Defects in the synthetic pathway prevent DIF-1 mediated stalk lineage specification cascade in the non-differentiating social amoeba, Acytostelium subglobosum
http://hdl.handle.net/2241/00123579
http://hdl.handle.net/2241/00123579480d233c-0216-48f1-b4e4-ceae17af475c
名前 / ファイル | ライセンス | アクション |
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BO_3-6 (799.7 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2015-03-04 | |||||
タイトル | ||||||
タイトル | Defects in the synthetic pathway prevent DIF-1 mediated stalk lineage specification cascade in the non-differentiating social amoeba, Acytostelium subglobosum | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Mohri, Kurato
× Mohri, Kurato× Hata, Takashi× Kikuchi, Haruhisa× Oshim, Yoshiteru× Urushihara, Hideko |
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著者別名 |
漆原, 秀子
× 漆原, 秀子 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Separation of somatic cells from germ-line cells is a crucial event for multicellular organisms, but how this step was achieved during evolution remains elusive. In Dictyostelium discoideum and many other dictyostelid species, solitary amoebae gather and form a multicellular fruiting body in which germ-line spores and somatic stalk cells differentiate, whereas in Acytostelium subglobosum, acellular stalks form and all aggregated amoebae become spores. In this study, because most D. discoideum genes known to be required for stalk cell differentiation have homologs in A. subglobosum, we inferred functional variations in these genes and examined conservation of the stalk cell specification cascade of D. discoideum mediated by the polyketide differentiation-inducing factor-1 (DIF-1) in A. subglobosum. Through heterologous expression of A. subglobosum orthologs of DIF-1 biosynthesis genes in D. discoideum, we confirmed that two of the three genes were functional equivalents, while DIF-methyltransferase (As-dmtA) involved at the final step of DIF-1 synthesis was not. In fact, DIF-1 activity was undetectable in A. subglobosum lysates and amoebae of this species were not responsive to DIF-1, suggesting a lack of DIF-1 production in this species. On the other hand, the molecular function of an A. subglobosum ortholog of DIF-1 responsive transcription factor was equivalent with that of D. discoideum and inhibition of polyketide synthesis caused developmental arrest in A. subglobosum, which could not be rescued by DIF-1 addition. These results suggest that non-DIF-1 polyketide cascades involving downstream transcription factors are required for fruiting body development of A. subglobosum. | |||||
書誌情報 |
Biology open 巻 3, 号 6, p. 553-560, 発行日 2014-06 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2046-6390 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 24876391 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1242/bio.20148359 | |||||
権利 | ||||||
権利情報 | © 2014. Published by The Company of Biologists Ltd | |||||
権利 | ||||||
権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | The Company of Biologists Ltd |