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ADF/Cofilin Is Not Essential but Is Critically Important for Actin Activities during Phagocytosis in Tetrahymena thermophila
http://hdl.handle.net/2241/121359
http://hdl.handle.net/2241/121359e16a899e-1932-419c-a129-a678428b7209
名前 / ファイル | ライセンス | アクション |
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EC_12-8.pdf (3.5 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2014-05-08 | |||||
タイトル | ||||||
タイトル | ADF/Cofilin Is Not Essential but Is Critically Important for Actin Activities during Phagocytosis in Tetrahymena thermophila | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Shiozaki, Nanami
× Shiozaki, Nanami× Nakano, Kentaro× Kushida, Yasuharu× Noguchi, Taro Q. P.× Uyeda, Taro Q. P.× Wloga, Dorota× Dave, Drashti× Vasudevan, Krishna Kumar× Gaertig, Jacek× Numata, Osamu |
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著者別名 |
中野, 賢太郎
× 中野, 賢太郎× 沼田, 治 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | ADF/cofilin is a highly conserved actin-modulating protein. Reorganization of the actin cytoskeleton in vivo through severing and depolymerizing of F-actin by this protein is essential for various cellular events, such as endocytosis, phagocytosis, cytokinesis, and cell migration. We show that in the ciliate Tetrahymena thermophila, the ADF/cofilin homologue Adf73p associates with actin on nascent food vacuoles. Overexpression of Adf73p disrupted the proper localization of actin and inhibited the formation of food vacuoles. In vitro, recombinant Adf73p promoted the depolymerization of filaments made of T. thermophila actin (Act1p). Knockout cells lacking the ADF73 gene are viable but grow extremely slowly and have a severely decreased rate of food vacuole formation. Knockout cells have abnormal aggregates of actin in the cytoplasm. Surprisingly, unlike the case in animals and yeasts, in Tetrahymena, ADF/cofilin is not required for cytokinesis. Thus, the Tetrahymena model shows promise for future studies of the role of ADF/cofilin in vivo. | |||||
書誌情報 |
Eukaryotic cell 巻 12, 号 8, p. 1080-1086, 発行日 2013-08 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1535-9778 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11642650 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1128/EC.00074-13 | |||||
権利 | ||||||
権利情報 | © 2013, American Society for Microbiology | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | American Society for Microbiology | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/121359 | |||||
識別子タイプ | HDL |