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Reaction pathway of tryptophanase-catalyzed L-tryptophan synthesis from D-serine
http://hdl.handle.net/2241/114816
http://hdl.handle.net/2241/1148161e8c263d-98fa-4ade-b621-82a8cc65e8d8
名前 / ファイル | ライセンス | アクション |
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JCB_879-29.pdf (632.6 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2012-01-13 | |||||
タイトル | ||||||
タイトル | Reaction pathway of tryptophanase-catalyzed L-tryptophan synthesis from D-serine | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Shimada, Akihiko
× Shimada, Akihiko× Ozaki, Haruka× Saito, Takeshi× Fujii, Noriko |
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著者別名 |
島田, 秋彦
× 島田, 秋彦 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Tryptophanase, L-tryptophan indole-lyase with extremely absolute stereospecificity, can change the stereospecificity in concentrated diammonium hydrogenphosphate solution. While tryptophanase is not inert to d-serine in the absence of diammonium hydrogenphosphate, it can undergo L-tryptophan synthesis from d-serine along with indole in the presence of it. It has been well known that tryptophanase synthesizes L-tryptophan from l-serine through a β-substitution mechanism of the ping-pong type. However, a metabolic pathway of L-tryptophan synthesis from d-serine has remained unclear. The present study aims to elucidate it. Diammonium hydrogenphosphate plays a role in the emergence of catalytic activity on d-serine. The salt gives tryptophanase a small conformational change, which makes it possible to catalyze d-serine. Tryptophanase-bound d-serine produces L-tryptophan synthesis by β-replacement reaction via the enzyme-bound aminoacrylate intermediate. Our result will be valuable in studying the origin of homochirality. | |||||
書誌情報 |
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 巻 879, 号 29, p. 3289-3295, 発行日 2011-11 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1570-0232 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11656178 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 21601540 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jchromb.2011.04.028 | |||||
権利 | ||||||
権利情報 | © 2011 Elsevier B.V. NOTICE: this is the author's version of a work that was accepted for publication in Journal of chromatography. B. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of chromatography. B, Vol.879 Issue 29, Pages: 3289-3295. doi:10.1016/j.jchromb.2011.04.028. |
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著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/114816 | |||||
識別子タイプ | HDL |