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Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter
http://hdl.handle.net/2241/114758
http://hdl.handle.net/2241/1147586eb35f0c-3d49-499b-9a5e-92c28531055b
名前 / ファイル | ライセンス | アクション |
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TR_20-6.pdf (4.7 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2011-12-09 | |||||
タイトル | ||||||
タイトル | Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Hirai, Tadayoshi
× Hirai, Tadayoshi× Kim, You-Wang× Kato, Kazuhisa× Hiwasa-Tanase, Kyoko× Ezura, Hiroshi |
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著者別名 |
江面, 浩
× 江面, 浩 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The E8 promoter, a tomato fruit-ripeningspecific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use. |
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書誌情報 |
Transgenic research 巻 20, 号 6, p. 1285-1292, 発行日 2011-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0962-8819 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10873487 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 21359850 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s11248-011-9495-9 | |||||
権利 | ||||||
権利情報 | © Springer Science+Business Media B.V. 2011. The final publication is available at www.springerlink.com |
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著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Springer | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/114758 | |||||
識別子タイプ | HDL |