WEKO3
アイテム
{"_buckets": {"deposit": "6614fa14-a7ce-49b1-baa7-7b6ad88b7e1b"}, "_deposit": {"id": "19392", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "19392"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00019392", "sets": ["1256"]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "473", "bibliographicPageStart": "469", "bibliographicVolumeNumber": "178", "bibliographic_titles": [{"bibliographic_title": "Plant science"}]}]}, "item_5_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "棚瀬, 京子"}], "nameIdentifiers": [{"nameIdentifier": "204525", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "00368672", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000000368672"}, {"nameIdentifier": "0000001311", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001311"}]}, {"creatorNames": [{"creatorName": "福田, 直也"}], "nameIdentifiers": [{"nameIdentifier": "204751", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80251023", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000080251023"}, {"nameIdentifier": "0000001271", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001271"}]}, {"creatorNames": [{"creatorName": "江面, 浩"}], "nameIdentifiers": [{"nameIdentifier": "169", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "00332552", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000000332552"}, {"nameIdentifier": "0000001297", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001297"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Miraculin is a taste-modifying protein that turns sour tastes into sweet ones. We previously generated transgenic tomato plants that constitutively expressed miraculin. To study the stability of transgene inheritance and expression in detail, three lines of transgenic tomato that highly accumulate miraculin in the T0 generation with a single copy of the miraculin gene were analyzed for genomic organization, mRNA expression and miraculin accumulation up to the T5 generation, corresponding to six generations of propagation. Transgenes were stably inherited and genomic rearrangement was not detected; this was confirmed in the T5 generation in one line and the T3 generation in the other two lines. The expression of miraculin mRNA was stable through multiple generations and in individual plants of the same generation. The concentrations of miraculin protein ranged from 8.73 to 11.52 μg/mg total soluble protein in the transgenic tomatoes, and they were stable in each line. These results suggest that the tomato is a suitable material for producing recombinant miraculin protein.", "subitem_description_type": "Abstract"}]}, "item_5_identifier_34": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2241/105326"}]}, "item_5_publisher_27": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier Ireland Ltd."}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.plantsci.2010.02.016", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2010 Elsevier Ireland Ltd."}]}, "item_5_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0168-9452", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10531279", "subitem_source_identifier_type": "NCID"}]}, "item_5_subject_20": {"attribute_name": "NIIサブジェクト", "attribute_value_mlt": [{"subitem_subject": "生物学", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yano, Megumu"}], "nameIdentifiers": [{"nameIdentifier": "69760", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirai, Tadayoshi"}], "nameIdentifiers": [{"nameIdentifier": "69761", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, Kazuhisa"}], "nameIdentifiers": [{"nameIdentifier": "69762", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hiwasa-Tanase, Kyoko"}], "nameIdentifiers": [{"nameIdentifier": "69763", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukuda, Naoya"}], "nameIdentifiers": [{"nameIdentifier": "69764", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ezura, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "69765", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-12-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "PS_178-5.pdf", "filesize": [{"value": "322.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 322000.0, "url": {"label": "PS_178-5.pdf", "url": "https://tsukuba.repo.nii.ac.jp/record/19392/files/PS_178-5.pdf"}, "version_id": "d63c4456-2624-4706-bdb0-a81452b61efd"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Tomato is a suitable material for producing recombinant miraculin protein in genetically stable manner", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Tomato is a suitable material for producing recombinant miraculin protein in genetically stable manner"}]}, "item_type_id": "5", "owner": "1", "path": ["528", "1256", "1257", "658"], "permalink_uri": "http://hdl.handle.net/2241/105326", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-06-16"}, "publish_date": "2010-06-16", "publish_status": "0", "recid": "19392", "relation": {}, "relation_version_is_last": true, "title": ["Tomato is a suitable material for producing recombinant miraculin protein in genetically stable manner"], "weko_shared_id": 5}
Tomato is a suitable material for producing recombinant miraculin protein in genetically stable manner
http://hdl.handle.net/2241/105326
http://hdl.handle.net/2241/10532620eff460-9ae6-48a3-9ccd-ef105ea3037f
名前 / ファイル | ライセンス | アクション |
---|---|---|
PS_178-5.pdf (322.0 kB)
|
|
Item type | Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2010-06-16 | |||||
タイトル | ||||||
タイトル | Tomato is a suitable material for producing recombinant miraculin protein in genetically stable manner | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Yano, Megumu
× Yano, Megumu× Hirai, Tadayoshi× Kato, Kazuhisa× Hiwasa-Tanase, Kyoko× Fukuda, Naoya× Ezura, Hiroshi |
|||||
著者別名 |
棚瀬, 京子
× 棚瀬, 京子× 福田, 直也× 江面, 浩 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Miraculin is a taste-modifying protein that turns sour tastes into sweet ones. We previously generated transgenic tomato plants that constitutively expressed miraculin. To study the stability of transgene inheritance and expression in detail, three lines of transgenic tomato that highly accumulate miraculin in the T0 generation with a single copy of the miraculin gene were analyzed for genomic organization, mRNA expression and miraculin accumulation up to the T5 generation, corresponding to six generations of propagation. Transgenes were stably inherited and genomic rearrangement was not detected; this was confirmed in the T5 generation in one line and the T3 generation in the other two lines. The expression of miraculin mRNA was stable through multiple generations and in individual plants of the same generation. The concentrations of miraculin protein ranged from 8.73 to 11.52 μg/mg total soluble protein in the transgenic tomatoes, and they were stable in each line. These results suggest that the tomato is a suitable material for producing recombinant miraculin protein. | |||||
書誌情報 |
Plant science 巻 178, 号 5, p. 469-473, 発行日 2010-05 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0168-9452 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10531279 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.plantsci.2010.02.016 | |||||
権利 | ||||||
権利情報 | © 2010 Elsevier Ireland Ltd. | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Elsevier Ireland Ltd. | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/105326 | |||||
識別子タイプ | HDL |