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Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions
http://hdl.handle.net/2241/00161424
http://hdl.handle.net/2241/00161424e29450a5-3eae-4d0c-b298-113669abd0fe
名前 / ファイル | ライセンス | アクション |
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IVCDBA_56-1 (2.9 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2020-09-18 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
西村, 健
× 西村, 健× Hamada, Atsuko× Akagi, Eri× Yamasaki, Sachiko× Nakatao, Hirotaka× Obayashi, Fumitaka× Ohtaka, Manami× Nakanishi, Mahito× Toratani, Shigeaki× Okamoto, Tetsuji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro. In addition, we evaluated microarray data from PBMC- and dental pulp–derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics. | |||||
書誌情報 |
en : In Vitro Cellular & Developmental Biology - Animal 巻 56, 号 1, p. 85-95, 発行日 2020-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1071-2690 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11003480 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s11626-019-00412-w | |||||
権利 | ||||||
権利情報 | © The Author(s) 2019 | |||||
権利 | ||||||
権利情報 | This article is distributed under the terms of the Creative Commons At tribution 4.0 International License (http:/ / creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Tissue Culture Association |