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Finding of Agr Phase Variants in Staphylococcus aureus
http://hdl.handle.net/2241/00161179
http://hdl.handle.net/2241/0016117935641cdf-28c3-4c43-840e-b40bdb443deb
名前 / ファイル | ライセンス | アクション |
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mBio_10-4 (2.3 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2020-09-04 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Finding of Agr Phase Variants in Staphylococcus aureus | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
大庭, 良介
× 大庭, 良介× 森川, 一也× Gor, Vishal× Takemura, Aya J.× Nishitani, Masami× Higashide, Masato× Medrano Romero, Veronica |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Staphylococcus aureus is an important human pathogen whose success is largely attributed to its vast arsenal of virulence factors that facilitate its invasion into, and survival within, the human host. The expression of these virulence factors is controlled by the quorum sensing accessory gene regulator (Agr) system. However, a large proportion of clinical S. aureus isolates are consistently found to have a mutationally inactivated Agr system. These mutants have a survival advantage in the host but are considered irreversible mutants. Here we show, for the first time, that a fraction of Agr-negative mutants can revert their Agr activity. By serially passaging Agr-negative strains and screening for phenotypic reversion of hemolysis and subsequent sequencing, we identified two mutational events responsible for reversion: a genetic duplication plus inversion event and a poly(A) tract alteration. Additionally, we demonstrate that one clinical Agr-negative methicillin-resistant S. aureus (MRSA) isolate could reproducibly generate Agr-revertant colonies with a poly(A) tract genetic mechanism. We also show that these revertants activate their Agr system upon phagocytosis. We propose a model in which a minor fraction of Agr-negative S. aureus strains are phase variants that can revert their Agr activity and may act as a cryptic insurance strategy against host-mediated stress. | |||||
書誌情報 |
en : mBio 巻 10, 号 4, p. e00796-19, 発行日 2019-07 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2150-7511 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 31387900 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1128/mBio.00796-19 | |||||
権利 | ||||||
権利情報 | © 2019 Gor et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license. | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | American Society for Microbiology |