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A novel cell lysis system induced by phosphate deficiency in the cyanobacterium Synechocystis sp. PCC 6803
http://hdl.handle.net/2241/00157248
http://hdl.handle.net/2241/00157248173fc058-e173-468d-87e5-907f55745ecb
名前 / ファイル | ライセンス | アクション |
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JAP_31-2 (1.4 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2019-07-30 | |||||
タイトル | ||||||
タイトル | A novel cell lysis system induced by phosphate deficiency in the cyanobacterium Synechocystis sp. PCC 6803 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
鈴木, 石根
× 鈴木, 石根× Asada, Ryo× Shiraiwa, Yoshihiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In the cultivation of microalgae for the production of useful compounds, cell disruption to extract the products of interest is a bottleneck process. To establish a cost-effective method to recover these cellular compounds, we developed a method to induce cell lysis via phosphate deficiency in the cyanobacterium Synechocystis sp. PCC 6803. In this system, the promoter from the phoA gene for alkaline phosphatase expressed bacteriophage genes encoding the lytic enzymes holin and endolysin, thus the cell lysis is induced under phosphate-deficient condition. We observed that 90% of the cells, introduced this bacteriophage genes, were lysed after 24 h of incubation under phosphate-deficient conditions. We also developed a method to induce cell lysis in highly concentrated cells for the efficient recovery of valuable cellular products and observed over 90% cell lysis after 16 h of incubation under these conditions. This inducible lysis system may contribute to decreased cell disruption costs in the algal biotechnology industry. | |||||
書誌情報 |
Journal of applied phycology 巻 31, 号 2, p. 1069-1076, 発行日 2019-04 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0921-8971 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10729877 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s10811-018-1652-6 | |||||
権利 | ||||||
権利情報 | © Springer Nature B.V. 2018 | |||||
権利 | ||||||
権利情報 | The final publication is available at link.springer.com | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Springer |