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Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors
http://hdl.handle.net/2241/00154155
http://hdl.handle.net/2241/001541551249da97-5329-402e-9235-2703c73e6723
名前 / ファイル | ライセンス | アクション |
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RT_9 (654.1 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2019-01-25 | |||||
タイトル | ||||||
タイトル | Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
西村, 健
× 西村, 健× Nishino, Koichiro× Arai, Yoshikazu× Takasawa, Ken× Toyoda, Masashi× Yamazaki-Inoue, Mayu× Sugawara, Tohru× Akutsu, Hidenori× Ohtaka, Manami× Nakanishi, Mahito× Umezawa, Akihiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Human induced pluripotent stem cells (iPSCs) are established by introducing several reprogramming factors, such as OCT3/4, SOX2, KLF4, c-MYC. Because of their pluripotency and immortality, iPSCs are considered to be a powerful tool for regenerative medicine. To date, iPSCs have been established all over the world by various gene delivery methods. All methods induced high-quality iPSCs, but epigenetic analysis of abnormalities derived from differences in the gene delivery methods has not yet been performed. Here, we generated genetically matched human iPSCs from menstrual blood cells by using three kinds of vectors, i.e., retrovirus, Sendai virus, and episomal vectors, and compared genome-wide DNA methylation profiles among them. Although comparison of aberrant methylation revealed that iPSCs generated by Sendai virus vector have lowest number of aberrant methylation sites among the three vectors, the iPSCs generated by non-integrating methods did not show vector-specific aberrant methylation. However, the differences between the iPSC lines were determined to be the number of random aberrant hypermethylated regions compared with embryonic stem cells. These random aberrant hypermethylations might be a cause of the differences in the properties of each of the iPSC lines. | |||||
書誌情報 |
Regenerative Therapy 巻 9, p. 71-78, 発行日 2018-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 23523204 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 30525077 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.reth.2018.08.002 | |||||
権利 | ||||||
権利情報 | © 2018, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Elsevier |