WEKO3
アイテム
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In multicellular structures of the group 4 dictyostelid Dictyostelium discoideum, GSK3 promotes spore over stalk-like differentiation. We investigated whether, similar to other sporulation-inducing genes such as cAMP-dependent protein kinase (PKA), this role of GSK3 is derived from an ancestral role in encystation of unicellular amoebas.\nResults\nWe deleted GSK3 in Polysphondylium pallidum, a group 2 dictyostelid which has retained encystation as an alternative survival strategy. Loss of GSK3 inhibited cytokinesis of cells in suspension, as also occurs in D. discoideum, but did not affect spore or stalk differentiation in P. pallidum. However, gsk3− amoebas entered into encystation under conditions that in wild type favour aggregation and fruiting body formation. The gsk3− cells were hypersensitive to osmolytes, which are known to promote encystation, and to cyst-inducing factors that are secreted during starvation. GSK3 was not itself regulated by these factors, but inhibited their effects.\nConclusions\nOur data show that GSK3 has a deeply conserved role in controlling cytokinesis, but not spore differentiation in Dictyostelia. Instead, in P. pallidum, one of many Dictyostelia that like their solitary ancestors can still encyst to survive starvation, GSK3 promotes multicellular development into fruiting bodies over unicellular encystment.\n", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "BioMed Central Ltd"}]}, "item_5_relation_10": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "29760875", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1186/s13227-018-0101-6", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© The Author(s) 2018. 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The Creative Commons Public Domain Dedication waiver (http://creat iveco mmons .org/ publi cdoma in/zero/1.0/) applies to the data made available in this article, unless otherwise stated."}]}, "item_5_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2041-9139", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kawabe, Yoshinori"}], "nameIdentifiers": [{"nameIdentifier": "192014", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Morio, Takahiro"}], "nameIdentifiers": [{"nameIdentifier": "192015", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanaka, Yoshimasa"}], "nameIdentifiers": [{"nameIdentifier": "192016", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Schaap, Pauline"}], "nameIdentifiers": [{"nameIdentifier": "192017", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-06-14"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ED_9-12.pdf", "filesize": [{"value": "8.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_6", "mimetype": "application/pdf", "size": 8700000.0, "url": {"label": "ED_9-12", "url": "https://tsukuba.repo.nii.ac.jp/record/46689/files/ED_9-12.pdf"}, "version_id": "cf14e8e2-9156-4ad6-8e43-056c8ff3ba77"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Glycogen synthase kinase 3 promotes multicellular development over unicellular encystation in encysting Dictyostelia", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Glycogen synthase kinase 3 promotes multicellular development over unicellular encystation in encysting Dictyostelia"}]}, "item_type_id": "5", "owner": "1", "path": ["517", "6762"], "permalink_uri": "http://hdl.handle.net/2241/00152017", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-06-14"}, "publish_date": "2018-06-14", "publish_status": "0", "recid": "46689", "relation": {}, "relation_version_is_last": true, "title": ["Glycogen synthase kinase 3 promotes multicellular development over unicellular encystation in encysting Dictyostelia"], "weko_shared_id": 5}
Glycogen synthase kinase 3 promotes multicellular development over unicellular encystation in encysting Dictyostelia
http://hdl.handle.net/2241/00152017
http://hdl.handle.net/2241/00152017cb253791-462a-42ca-a0eb-c16f78ba7b41
名前 / ファイル | ライセンス | アクション |
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ED_9-12 (8.7 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2018-06-14 | |||||
タイトル | ||||||
タイトル | Glycogen synthase kinase 3 promotes multicellular development over unicellular encystation in encysting Dictyostelia | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Kawabe, Yoshinori
× Kawabe, Yoshinori× Morio, Takahiro× Tanaka, Yoshimasa× Schaap, Pauline |
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著者別名 |
森尾, 貴広
× 森尾, 貴広 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background Glycogen synthase kinase 3 (GSK3) regulates many cell fate decisions in animal development. In multicellular structures of the group 4 dictyostelid Dictyostelium discoideum, GSK3 promotes spore over stalk-like differentiation. We investigated whether, similar to other sporulation-inducing genes such as cAMP-dependent protein kinase (PKA), this role of GSK3 is derived from an ancestral role in encystation of unicellular amoebas. Results We deleted GSK3 in Polysphondylium pallidum, a group 2 dictyostelid which has retained encystation as an alternative survival strategy. Loss of GSK3 inhibited cytokinesis of cells in suspension, as also occurs in D. discoideum, but did not affect spore or stalk differentiation in P. pallidum. However, gsk3− amoebas entered into encystation under conditions that in wild type favour aggregation and fruiting body formation. The gsk3− cells were hypersensitive to osmolytes, which are known to promote encystation, and to cyst-inducing factors that are secreted during starvation. GSK3 was not itself regulated by these factors, but inhibited their effects. Conclusions Our data show that GSK3 has a deeply conserved role in controlling cytokinesis, but not spore differentiation in Dictyostelia. Instead, in P. pallidum, one of many Dictyostelia that like their solitary ancestors can still encyst to survive starvation, GSK3 promotes multicellular development into fruiting bodies over unicellular encystment. |
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書誌情報 |
EvoDevo 巻 9, 号 1, p. 12, 発行日 2018-05 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2041-9139 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 29760875 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/s13227-018-0101-6 | |||||
権利 | ||||||
権利情報 | © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat iveco mmons .org/licen ses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creat iveco mmons .org/ publi cdoma in/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | BioMed Central Ltd |