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Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302
http://hdl.handle.net/2241/00148438
http://hdl.handle.net/2241/00148438de5688b7-e24a-491b-adf0-2cda6436ff66
名前 / ファイル | ライセンス | アクション |
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SCResearch_23 (1.1 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-10-10 | |||||
タイトル | ||||||
タイトル | Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Nishimura, Ken
× Nishimura, Ken× Ohtaka, Manami× Takada, Hitomi× Kurisaki, Akira× Tran, Nhi Vo Kieu× Tran, Yen Thi Hai× Hisatake, Koji× Sano, Masayuki× Nakanishi, Mahito |
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著者別名 |
西村, 健
× 西村, 健× 久武, 幸司 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Transgene-free induced pluripotent stem cells (iPSCs) are valuable for both basic research and potential clinical applications. We previously reported that a replication-defective and persistent Sendai virus (SeVdp) vector harboring four reprogramming factors (SeVdp-iPS) can efficiently induce generation of transgene-free iPSCs. This vector can express all four factors stably and simultaneously without chromosomal integration and can be eliminated completely from reprogrammed cells by suppressing vector-derived RNA-dependent RNA polymerase. Here, we describe an improved SeVdp-iPS vector (SeVdp(KOSM)302L) that is automatically erased in response to microRNA-302 (miR-302), uniquely expressed in pluripotent stem cells (PSCs). Gene expression and genome replication of the SeVdp-302L vector, which contains miRNA-302a target sequences at the 3′ untranslated region of L mRNA, are strongly suppressed in PSCs. Consequently, SeVdp(KOSM)302L induces expression of reprogramming factors in somatic cells, while it is automatically erased from cells successfully reprogrammed to express miR-302. As this vector can reprogram somatic cells into transgene-free iPSCs without the aid of exogenous short interfering RNA (siRNA), the results we present here demonstrate that this vector may become an invaluable tool for the generation of human iPSCs for future clinical applications. | |||||
書誌情報 |
Stem Cell Research 巻 23, p. 13-19, 発行日 2017-08 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18735061 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 28666145 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.scr.2017.06.011 | |||||
権利 | ||||||
権利情報 | © 2017 The Authors. Published by Elsevier B.V. | |||||
権利 | ||||||
権利情報 | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Elsevier |