WEKO3
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Molecular dissection of the actin-binding ability of the fission yeast α-actinin, Ain1, in vitro and in vivo
http://hdl.handle.net/2241/00148356
http://hdl.handle.net/2241/0014835604bd84d2-f9fa-4eee-bfea-0f5610db1059
名前 / ファイル | ライセンス | アクション |
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JOB_162-2 (5.6 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-09-27 | |||||
タイトル | ||||||
タイトル | Molecular dissection of the actin-binding ability of the fission yeast α-actinin, Ain1, in vitro and in vivo | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Morita, Rikuri
× Morita, Rikuri× Takaine, Masak× Numata, Osamu× Nakano, Kentaro |
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著者別名 |
中野, 賢太郎
× 中野, 賢太郎× 沼田, 治 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A contractile ring (CR) is involved in cytokinesis in animal and yeast cells. Although several types of actin-bundling proteins associate with F-actin in the CR, their individual roles in the CR have not yet been elucidated in detail. Ain1 is the sole α-actinin homologue in the fission yeast Schizosaccharomyces pombe and specifically localizes to the CR with a high turnover rate. S. pombe cells lacking the ain1+ gene show defects in cytokinesis under stress conditions. We herein investigated the biochemical activity and cellular localization mechanisms of Ain1. Ain1 showed weaker affinity to F-actin in vitro than other actin-bundling proteins in S. pombe. We identified a mutation that presumably loosened the interaction between two calponin-homology domains constituting the single actin-binding domain (ABD) of Ain1, which strengthened the actin-binding activity of Ain1. This mutant protein induced a deformation in the ring shape of the CR. Neither a truncated protein consisting only of an N-terminal ABD nor a truncated protein lacking a C-terminal region containing an EF-hand motif localized to the CR, whereas the latter was involved in the bundling of F-actin in vitro. We herein propose detailed mechanisms for how each part of the molecule is involved in the proper cellular localization and function of Ain1. | |||||
書誌情報 |
The journal of biochemistry 巻 162, 号 2, p. 93-102, 発行日 2017-08 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0021-924X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00694073 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1093/jb/mvx008 | |||||
権利 | ||||||
権利情報 | © The Authors 2017.Published by Oxford University Press on behalf of the Japanese Biochemical Society. | |||||
権利 | ||||||
権利情報 | This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Biochemistry following peer review. The version of record J Biochem (2017) 162 (2): 93–102 is available online at: https://doi.org/10.1093/jb/mvx008. | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Oxford University Press |