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While ceramide is known to be an inducer of apoptosis of cochlear hair cells in cisplatin ototoxicity, little is known about the function of C1P in cochlear diseases.\n\nPurpose\nThe present study was designed to examine whether C1P could protect cochlear hair cells against cisplatin ototoxicity.\n\nMaterials and methods\nExplants of cochlear basal turns collected from C57BL/6J mice at postnatal days 3–5 were used in all experiments. Cochlear explants were exposed to 5 or 10 μM cisplatin for 48 h to assess the effects of C1P, NVP-231 (a ceramide kinase inhibitor), or ceramide. Western blotting of pAkt/Akt and pMAPK/MAPK was examined to check whether this pathway was modulated by C1P.\n\nResults\nC1P activated the Akt and MAPK pathway and significantly reduced cochlear cell death induced by cisplatin. Coadministration of cisplatin and ceramide significantly increased cochlear hair cell death. In addition, when treating cochlear hair cells with NVP-231 in the presence of cisplatin or ceramide, a remarkable increase in apoptosis of hair cells was observed.\n\nConclusion\nThe present findings confirmed the protective effects of C1P in the cisplatin ototoxicity. 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Ceramide-1-phosphate protection of cochlear hair cells against cisplatin ototoxicity
http://hdl.handle.net/2241/00146066
http://hdl.handle.net/2241/0014606632f74411-cee9-4abc-ba14-354181921704
名前 / ファイル | ライセンス | アクション |
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TR_3-450 (2.3 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-05-11 | |||||
タイトル | ||||||
タイトル | Ceramide-1-phosphate protection of cochlear hair cells against cisplatin ototoxicity | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Le, Quang
× Le, Quang× Tabuchi, Keiji× Hara, Akira |
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著者別名 |
田渕, 経司
× 田渕, 経司× 原, 晃 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background Ceramide-1-phosphate (C1P) is a phosphorylated form of ceramide. While ceramide is known to be an inducer of apoptosis of cochlear hair cells in cisplatin ototoxicity, little is known about the function of C1P in cochlear diseases. Purpose The present study was designed to examine whether C1P could protect cochlear hair cells against cisplatin ototoxicity. Materials and methods Explants of cochlear basal turns collected from C57BL/6J mice at postnatal days 3–5 were used in all experiments. Cochlear explants were exposed to 5 or 10 μM cisplatin for 48 h to assess the effects of C1P, NVP-231 (a ceramide kinase inhibitor), or ceramide. Western blotting of pAkt/Akt and pMAPK/MAPK was examined to check whether this pathway was modulated by C1P. Results C1P activated the Akt and MAPK pathway and significantly reduced cochlear cell death induced by cisplatin. Coadministration of cisplatin and ceramide significantly increased cochlear hair cell death. In addition, when treating cochlear hair cells with NVP-231 in the presence of cisplatin or ceramide, a remarkable increase in apoptosis of hair cells was observed. Conclusion The present findings confirmed the protective effects of C1P in the cisplatin ototoxicity. The balance between ceramide and C1P may play a critical role in the determination of hair cell fate in cisplatin ototoxicity. |
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書誌情報 |
Toxicology reports 巻 3, p. 450-457, 発行日 2016 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 22147500 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.toxrep.2016.04.003 | |||||
権利 | ||||||
権利情報 | © 2016 Published by Elsevier Ireland Ltd. | |||||
権利 | ||||||
権利情報 | This is an open access article under the CC BY-NC-NDlicense (http://creativecommons.org/licenses/by-nc-nd/4.0/). | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Elesevier |