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Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper-spectral coherent anti-Stokes Raman microscopy using a sub-nanosecond supercontinuum laser source
http://hdl.handle.net/2241/00145532
http://hdl.handle.net/2241/001455325e79f38e-8b72-45ca-b04a-60c93dcd602d
名前 / ファイル | ライセンス | アクション |
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JRS_48-1 (4.2 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-03-14 | |||||
タイトル | ||||||
タイトル | Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper-spectral coherent anti-Stokes Raman microscopy using a sub-nanosecond supercontinuum laser source | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Ishitsuka, Kei
× Ishitsuka, Kei× Koide, Masahiro× Yoshida, Masaki× Segawa, Hiroki× Leproux, Philippe× Couderc, Vincent× Watanabe, Makoto M.× Kano, Hideaki |
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著者別名 |
吉田, 昌樹
× 吉田, 昌樹× 加納, 英明 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We applied hyper-spectral coherent anti-Stokes Raman scattering imaging to intracellular lipid identification in living microalgae, Aurantiochytrium mangrovei 18W-13a. Two different lipids, squalene and triacylglycerol, were found inside living cells with clear vibrational contrast. Based on the endogenous lipid band as a result of the cis C[DOUBLE BOND]C stretch vibrational mode, squalene and triacylglycerol were clearly distinguished in different intracellular areas. In particular, squalene was detected solely in vacuoles as lipid particles, which was also supported by electron microscopy. | |||||
書誌情報 |
Journal of Raman Spectroscopy 巻 48, 号 1, p. 8-15, 発行日 2017-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03770486 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00705861 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1002/jrs.4979 | |||||
権利 | ||||||
権利情報 | © 2016 John Wiley & Sons, Ltd. | |||||
権利 | ||||||
権利情報 | This is the peer reviewed version of the following article: http://onlinelibrary.wiley.com/doi/10.1002/jrs.4979/abstract, which has been published in final form at 10.1002/jrs.4979. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Wiley |