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Conservation of the Nrf2-Mediated Gene Regulation of Proteasome Subunits and Glucose Metabolism in Zebrafish
http://hdl.handle.net/2241/00145311
http://hdl.handle.net/2241/0014531151d6c540-3d74-432d-97a6-f83921e1ac2b
名前 / ファイル | ライセンス | アクション |
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OMCL_2016-5720574 (2.5 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-02-17 | |||||
タイトル | ||||||
タイトル | Conservation of the Nrf2-Mediated Gene Regulation of Proteasome Subunits and Glucose Metabolism in Zebrafish | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Nguyen, Vu Thanh
× Nguyen, Vu Thanh× Fuse, Yuji× Tamaoki, Junya× Akiyama, Shin-ichi× Muratani, Masafumi× Tamaru, Yutaka× Kobayashi, Makoto |
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著者別名 |
村谷, 匡史
× 村谷, 匡史× 小林, 麻己人 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The Keap1-Nrf2 system is an evolutionarily conserved defense mechanism against oxidative and xenobiotic stress. Besides the exogenous stress response, Nrf2 has been found to regulate numerous cellular functions, including protein turnover and glucose metabolism; however, the evolutionary origins of these functions remain unknown. In the present study, we searched for novel target genes associated with the zebrafish Nrf2 to answer this question. A microarray analysis of zebrafish embryos that overexpressed Nrf2 revealed that 115 candidate genes were targets of Nrf2, including genes encoding proteasome subunits and enzymes involved in glucose metabolism. A real-time quantitative PCR suggested that the expression of 3 proteasome subunits (psma3, psma5, and psmb7) and 2 enzymes involved in glucose metabolism (pgd and fbp1a) were regulated by zebrafish Nrf2. We thus next examined the upregulation of these genes by an Nrf2 activator, diethyl maleate, using Nrf2 mutant zebrafish larvae. The results of real-time quantitative PCR and whole-mount in situ hybridization showed that all of these 5 genes were upregulated by diethyl maleate treatment in an Nrf2-dependent manner, especially in the liver. These findings implied that the Nrf2-mediated regulation of the proteasome subunits and glucose metabolism is evolutionarily conserved among vertebrates. | |||||
書誌情報 |
Oxidative medicine and cellular longevity 巻 2016, p. 5720574, 発行日 2016 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1942-0900 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12489832 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 28116036 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1155/2016/5720574 | |||||
権利 | ||||||
権利情報 | © 2016 Vu Thanh Nguyen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Hindawi Publishing Corporation |