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Morphological and Mechanical Properties of Osteosarcoma Microenvironment Cells Explored by Atomic Force Microscopy
http://hdl.handle.net/2241/00144986
http://hdl.handle.net/2241/00144986822cde4f-deb6-4e0d-8339-b8f3163a194e
名前 / ファイル | ライセンス | アクション |
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AS_32-11 (1.1 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-01-18 | |||||
タイトル | ||||||
タイトル | Morphological and Mechanical Properties of Osteosarcoma Microenvironment Cells Explored by Atomic Force Microscopy | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
WANG, Xinlong
× WANG, Xinlong× YANG, Yingjun× HU, Xiaohong× KAWAZOE, Naoki× YANG, Yingnan× CHEN, Guoping |
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著者別名 |
楊, 英男
× 楊, 英男 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cell mechanical properties that depend on cytoskeleton architecture are critical to the mechanotransduction process, and have great potential for cancer diagnosis and therapy. In this study, the morphological and mechanical properties of typical osteosarcoma microenvironment cells, including mesenchymal stem cells (MSC), normal human osteoblast cells (NHOst) and osteosarcoma cells (MG-63), were compared using atomic force microscopy (AFM). The MG-63 cells were smaller and thicker than the MSC and NHOst cells. The membrane roughness of MG-63 cells was higher than that of MSC and NHOst cells. The MG-63 cells had lower stiffness than their normal counterparts due to their reduced organization of the cytoskeleton structure. The cell stiffness influenced the mechanotransduction. The MG-63 cells had a lower percentage of nuclear YAP/TAZ compared with the MSC and NHOst cells. The F-actin assembly was disrupted by the cytochalasin D (cyto D) treatment used to investigate its influence on mechanotransduction. Disruption of the cytoskeleton leaded to a decrease of the cell stiffness, and reduced the nuclear YAP/TAZ percentage, indicating its inhibition in the cell mechanotransduction process. This study would shed light on the development of a novel cancer diagnosis strategy and would contribute to reveal the relationship between the cytoskeleton structure and the cell mechanical properties. | |||||
書誌情報 |
Analytical sciences 巻 32, 号 11, p. 1177-1182, 発行日 2016-11 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0910-6340 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10500785 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 27829622 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2116/analsci.32.1177 | |||||
権利 | ||||||
権利情報 | 2016 © The Japan Society for Analytical Chemistry | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | The Japan Society for Analytical Chemistry |