WEKO3
アイテム
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C-terminal acidic domain of histone chaperone human NAP1 is an efficient binding assistant for histone H2A-H2B, but not H3-H4
http://hdl.handle.net/2241/00141562
http://hdl.handle.net/2241/00141562cf91f5f5-2830-4889-b835-61e700aad4ae
名前 / ファイル | ライセンス | アクション |
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GC_21-3 (1.1 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2016-05-23 | |||||
タイトル | ||||||
タイトル | C-terminal acidic domain of histone chaperone human NAP1 is an efficient binding assistant for histone H2A-H2B, but not H3-H4 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Ohtomo, Hideaki
× Ohtomo, Hideaki× Akashi, Satoko× Moriwaki, Yoshihito× Okuwaki, Mitsuru× Osakabe, Akihisa× Nagata, Kyosuke× Kurumizaka, Hitoshi× Nishimura, Yoshifumi |
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著者別名 |
奧脇, 暢
× 奧脇, 暢× 永田, 恭介 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Nucleosome assembly protein 1 (NAP1) binds both the (H3-H4)2 tetramer and two H2A-H2B dimers, mediating their sequential deposition on DNA. NAP1 contains a C-terminal acidic domain (CTAD) and a core domain that promotes dimer formation. Here, we have investigated the roles of the core domain and CTAD of human NAP1 in binding to H2A-H2B and H3-H4 by isothermal calorimetry and native mass spectrometry and compared them with the roles of yeast NAP1. We show that the hNAP1 and yNAP1 dimers bind H2A-H2B by two different modes: a strong endothermic interaction and a weak exothermic interaction. A mutant hNAP1, but not yNAP1, dimer lacking CTAD loses the exothermic interaction and shows greatly reduced H2A-H2B binding activity. The isolated CTAD of hNAP1 binds H2A-H2B only exothermically with relatively stronger binding as compared with the exothermic interaction observed for the full-length hNAP1 dimer. Thus, the two CTADs in the hNAP1 dimer seem to provide binding assistance for the strong endothermic interaction of the core domain with H2A-H2B. By contrast, in the relatively weaker binding of hNAP1 to H3-H4 as compared with yNAP1, CTAD of hNAP1 has no significant role. To our knowledge, this is the first distinct role identified for the hNAP1 CTAD. | |||||
書誌情報 |
Genes to cells 巻 21, 号 3, p. 252-263, 発行日 2016-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1356-9597 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11078945 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 26841755 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/gtc.12339 | |||||
権利 | ||||||
権利情報 | © 2016 The Authors Genes to Cells published by Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd. | |||||
権利 | ||||||
権利情報 | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Wiley |