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Expansion of mouse hematopoietic progenitor cells in three-dimensional cocultures on frozen-thawed stromal cell layers formed within porous scaffolds
http://hdl.handle.net/2241/00124386
http://hdl.handle.net/2241/00124386ada20900-d08e-4eb6-9a95-003f210bd2e4
名前 / ファイル | ライセンス | アクション |
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EH_43-2 (278.7 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2015-04-23 | |||||
タイトル | ||||||
タイトル | Expansion of mouse hematopoietic progenitor cells in three-dimensional cocultures on frozen-thawed stromal cell layers formed within porous scaffolds | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Miyoshi, Hirotoshi
× Miyoshi, Hirotoshi× Morita, Misa× Ohshima, Norio× Sato, Chiaki |
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著者別名 |
三好, 浩稔
× 三好, 浩稔 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To establish a highly efficient method of ex vivo expansion of hematopoietic cells (HCs), three-dimensional (3D) cocultures of HCs and stromal cell lines were performed using porous polymer scaffolds. Hematopoietic cells derived from mouse fetal livers were expanded by two successive cultures without the use of exogenous cytokines, namely, 3D cultures of stromal cells (DAS 104-8 cell line) to form stromal layers within the scaffolds, and, subsequently, by cocultures of the HCs on the stromal cell layers for 2 weeks. To expand the HCs more conveniently, in some experiments the stromal layers formed within the scaffolds were frozen (3D freezing) before the cocultures, then stored and applied to the cocultures after thawing. When the HCs were cocultured on the stromal layers of the DAS 104-8 cells, primitive HCs (c-kit+ and CD34+ cells) were expanded several fold during the cocultures. In contrast, the expansion of these primitive HCs was remarkably enhanced in the cocultures using the 3D frozen-thawed DAS 104-8 stromal layers (c-kit+ cells > fifteenfold and CD34+ cells > thirtyfold), and these expansions were significantly higher than those without the 3D freezing. The expansions enhanced by cocultures on the 3D frozen-thawed stromal layers were also observed in the cocultures with another stromal cell line (DAS 104-4). Because 3D frozen-thawed stromal cell lines are easy to handle, 3D coculture of HCs on frozen-thawed stromal cell lines may be an effective and convenient method for expanding primitive HCs. | |||||
書誌情報 |
Experimental hematology 巻 43, 号 2, p. 115-124, 発行日 2015-02 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0301-472X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00641088 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 25461256 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.exphem.2014.08.008 | |||||
権利 | ||||||
権利情報 | © 2015 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc. NOTICE: this is the author’s version of a work that was accepted for publication in Experimental Hematology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Experimental Hematology, 43, 2, (2015). doi:10.1016/j.exphem.2014.08.008 | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | International Society for Experimental Hematology |