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Synergistic solubilization of porcine myosin in physiological salt solution by arginine
http://hdl.handle.net/2241/120870
http://hdl.handle.net/2241/1208706eb35208-4cca-4612-807f-41843f985b72
名前 / ファイル | ライセンス | アクション |
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IJBM_62.pdf (2.4 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2014-02-28 | |||||
タイトル | ||||||
タイトル | Synergistic solubilization of porcine myosin in physiological salt solution by arginine | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
白木, 賢太郎
× 白木, 賢太郎 |
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著者別名 |
白木, 賢太郎
× 白木, 賢太郎 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Myosin is an important protein resource for food industries and has a bipolar filamentous structure that is composed of subfilaments that occur in vivo. It has been shown that a high ionic strength is required to prevent myosin from forming filamentous structures and to solubilize the protein in aqueous solution. In the presence of 100–200 mM NaCl, 50 mM arginine was more effective than other additives tested, including NaCl, in myosin solubilization. Before reaching equilibrium solubility, the myosin solution was initially supersaturated upon the dilution of a stock myosin solution in 1 M NaCl into the test solvents. Arginine slowed the process of equilibration and stabilized the supersaturated solution more effectively than other additives. No structural changes in myosin caused by arginine were observed, which indicated that arginine enhanced the solubility of myosin in a physiological salt solution without affecting the structure. | |||||
書誌情報 |
International journal of biological macromolecules 巻 62, p. 647-651, 発行日 2013-10 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0141-8130 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1153092X | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 24095712 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ijbiomac.2013.09.035 | |||||
権利 | ||||||
権利情報 | © 2013 Elsevier B.V. NOTICE: this is the author’s version of a work that was accepted for publication in International Journal of Biological Macromolecules. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Journal of Biological Macromolecules, 62, 2013, http://dx.doi.org/10.1016/j.ijbiomac.2013.09.035. | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | ELSEVIER | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/120870 | |||||
識別子タイプ | HDL |