WEKO3
アイテム
{"_buckets": {"deposit": "029e7494-30ae-4733-be02-19e67e73eacc"}, "_deposit": {"id": "27427", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "27427"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00027427", "sets": ["1443", "1879"]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "1328", "bibliographicPageStart": "1317", "bibliographicVolumeNumber": "84", "bibliographic_titles": [{"bibliographic_title": "Pure and applied chemistry"}]}]}, "item_5_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "北, 将樹"}], "nameIdentifiers": [{"nameIdentifier": "643", "nameIdentifierScheme": "WEKO"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Venomous mammals are rare, and only a few species in the orders Insectivora and Monotremata produce toxic venom. Among them, the duckbill platypus (Ornithorhynchus anatinus) is one of the two venomous Australian mammals. The adult male platypus carries a spur on each hind leg, which it uses to inject competitors with poison. However, the structure and function of the poison’s active compounds are still imcompletely characterized. We found that crude platypus venom produced potent Ca2+ influx in human neuroblastoma IMR‑32 cells. Guided by this assay, we identified 11 unique peptides, including peptide H–His–Asp–His–Pro–Asn–Pro–Arg–OH, which coincided with the N-terminal domain residues of Ornithorhynchus venom C-type natriuretic peptide (OvCNP). This heptapeptide induced a significant increase in [Ca2+]i in IMR-32 cells at 75 μM; had relatively specific affinities for glutamate, histamine, and GABAA receptors; and facilitated neurogenic twitching in guinea pig ileum specimens at 30 μM. We also established that its proteinous venom fraction strongly hydrolyzed Pro–Phe–Arg–MCA and cleaved a human low-molecular-weight kininogen (LK), similar to porcine pancreas kallikrein. These results strongly indicated that platypus venom contains tissue kallikrein-like protease(s), and its proteolytic activity might synergistically contribute to toxicity through the specific cleavage of other venom constituents.", "subitem_description_type": "Abstract"}]}, "item_5_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Conference:27th International Symposium on the Chemistry of Natural Products and the 7th International Conference on Biodiversity (ISCNP-27 \u0026 ICOB-7), Brisbane, Australia, 10–15 July 2011", "subitem_description_type": "Other"}]}, "item_5_identifier_34": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2241/117287"}]}, "item_5_publisher_27": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "International Union of Pure and Applied Chemistry"}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1351/PAC-CON-11-08-18", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2012 IUPAC"}]}, "item_5_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0033-4545", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00797859", "subitem_source_identifier_type": "NCID"}]}, "item_5_subject_20": {"attribute_name": "NIIサブジェクト", "attribute_value_mlt": [{"subitem_subject": "基礎工学・応用物理学", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kita, Masaki"}], "nameIdentifiers": [{"nameIdentifier": "94721", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-12-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "PAC_84-6.pdf", "filesize": [{"value": "719.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 719600.0, "url": {"label": "PAC_84-6.pdf", "url": "https://tsukuba.repo.nii.ac.jp/record/27427/files/PAC_84-6.pdf"}, "version_id": "2b80dfa7-38ac-41e4-9160-a49b3f4fbc50"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Bioorganic studies on the venom from duckbill platypus", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Bioorganic studies on the venom from duckbill platypus"}]}, "item_type_id": "5", "owner": "1", "path": ["1443", "1879"], "permalink_uri": "http://hdl.handle.net/2241/117287", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-06-13"}, "publish_date": "2012-06-13", "publish_status": "0", "recid": "27427", "relation": {}, "relation_version_is_last": true, "title": ["Bioorganic studies on the venom from duckbill platypus"], "weko_shared_id": 5}
Bioorganic studies on the venom from duckbill platypus
http://hdl.handle.net/2241/117287
http://hdl.handle.net/2241/117287dae2f47c-e4f5-4e1e-a95b-3da297f0e798
名前 / ファイル | ライセンス | アクション |
---|---|---|
PAC_84-6.pdf (719.6 kB)
|
|
Item type | Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2012-06-13 | |||||
タイトル | ||||||
タイトル | Bioorganic studies on the venom from duckbill platypus | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Kita, Masaki
× Kita, Masaki |
|||||
著者別名 |
北, 将樹
× 北, 将樹 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Venomous mammals are rare, and only a few species in the orders Insectivora and Monotremata produce toxic venom. Among them, the duckbill platypus (Ornithorhynchus anatinus) is one of the two venomous Australian mammals. The adult male platypus carries a spur on each hind leg, which it uses to inject competitors with poison. However, the structure and function of the poison’s active compounds are still imcompletely characterized. We found that crude platypus venom produced potent Ca2+ influx in human neuroblastoma IMR‑32 cells. Guided by this assay, we identified 11 unique peptides, including peptide H–His–Asp–His–Pro–Asn–Pro–Arg–OH, which coincided with the N-terminal domain residues of Ornithorhynchus venom C-type natriuretic peptide (OvCNP). This heptapeptide induced a significant increase in [Ca2+]i in IMR-32 cells at 75 μM; had relatively specific affinities for glutamate, histamine, and GABAA receptors; and facilitated neurogenic twitching in guinea pig ileum specimens at 30 μM. We also established that its proteinous venom fraction strongly hydrolyzed Pro–Phe–Arg–MCA and cleaved a human low-molecular-weight kininogen (LK), similar to porcine pancreas kallikrein. These results strongly indicated that platypus venom contains tissue kallikrein-like protease(s), and its proteolytic activity might synergistically contribute to toxicity through the specific cleavage of other venom constituents. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Conference:27th International Symposium on the Chemistry of Natural Products and the 7th International Conference on Biodiversity (ISCNP-27 & ICOB-7), Brisbane, Australia, 10–15 July 2011 | |||||
書誌情報 |
Pure and applied chemistry 巻 84, 号 6, p. 1317-1328, 発行日 2012-04 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0033-4545 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00797859 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1351/PAC-CON-11-08-18 | |||||
権利 | ||||||
権利情報 | © 2012 IUPAC | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | International Union of Pure and Applied Chemistry | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/117287 | |||||
識別子タイプ | HDL |