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Novel redox nanomedicine improves gene expression of polyion complex vector
http://hdl.handle.net/2241/115376
http://hdl.handle.net/2241/11537647eed4ac-e877-4925-a9d2-28c68aeb8774
名前 / ファイル | ライセンス | アクション |
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STAM_12-6.pdf (708.0 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2012-02-27 | |||||
タイトル | ||||||
タイトル | Novel redox nanomedicine improves gene expression of polyion complex vector | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Toh, Kazuko
× Toh, Kazuko× Yoshitomi, Toru× Ikeda, Yutaka× Nagasaki, Yukio |
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著者別名 |
池田, 豊
× 池田, 豊× 長崎, 幸夫 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Gene therapy has generated worldwide attention as a new medical technology. While non-viral gene vectors are promising candidates as gene carriers, they have several issues such as toxicity and low transfection efficiency. We have hypothesized that the generation of reactive oxygen species (ROS) affects gene expression in polyplex supported gene delivery systems. The effect of ROS on the gene expression of polyplex was evaluated using a nitroxide radical-containing nanoparticle (RNP) as an ROS scavenger. When polyethyleneimine (PEI)/pGL3 or PEI alone was added to the HeLa cells, ROS levels increased significantly. In contrast, when (PEI)/pGL3 or PEI was added with RNP, the ROS levels were suppressed. The luciferase expression was increased by the treatment with RNP in a dose-dependent manner and the cellular uptake of pDNA was also increased. Inflammatory cytokines play an important role in ROS generation in vivo. In particular, tumor necrosis factor (TNF)-α caused intracellular ROS generation in HeLa cells and decreased gene expression. RNP treatment suppressed ROS production even in the presence of TNF-α and increased gene expression. This anti-inflammatory property of RNP suggests that it may be used as an effective adjuvant for non-viral gene delivery systems. | |||||
書誌情報 |
Science and technology of advanced materials 巻 12, 号 6, p. 065001, 発行日 2011 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1468-6996 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11539797 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1088/1468-6996/12/6/065001 | |||||
権利 | ||||||
権利情報 | © 2011 National Institute for Materials Science | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | IOP PUBLISHING | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/115376 | |||||
識別子タイプ | HDL |