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Differential roles of calcitonin family peptides in the dendrite formation and spinogenesis of the cerebral cortex in vitro
http://hdl.handle.net/2241/114622
http://hdl.handle.net/2241/114622b59db59f-ab79-4c42-9996-76c7de236b52
名前 / ファイル | ライセンス | アクション |
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NP_45-4.pdf (47.5 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2011-11-15 | |||||
タイトル | ||||||
タイトル | Differential roles of calcitonin family peptides in the dendrite formation and spinogenesis of the cerebral cortex in vitro | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Harigai, Yuichi
× Harigai, Yuichi× Natsume, Masaki× Li, Fei× Ohtani, Akiko× Senzaki, Kouji× Shiga, Takashi |
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著者別名 |
先崎, 浩次
× 先崎, 浩次× 志賀, 隆 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We examined roles of calcitonin family peptides in the initial stages of dendrite formation and the maturation of dendritic spines in the rat cerebral cortex in vitro. Embryonic day 18 cortical neurons were dissociated and cultured for 2–3 days in the presence of calcitonin gene-related peptide (CGRP), calcitonin, amylin or adrenomedullin. The treatment of cortical neurons with CGRP promoted the formation of primary dendrites of non-GABAergic neurons. In contrast, the treatment with amylin and adrenomedullin for 3 days inhibited the dendritic elongation of non-GABAergic neurons. Calcitonin had no effect on the initial dendrite formation. Next, we examined roles of the peptides in the spine formation. Embryonic day 16 cortical neurons were cultured for 14 days and then treated acutely with CGRP, amylin or adrenomedullin for 24 h. The density of filopodia, puncta/stubby spines and spines were increased by the CGRP treatment, whereas decreased by amylin. Therefore, CGRP and amylin showed opposite effects on the formation of dendritic filopodia, puncta and spines. Adrenomedullin had no effects on the spine formation. In conclusion, the present study showed that calcitonin family peptides have differential effects both in the dendrite formation during the initial stages and the spine formation of cortical neurons in vitro. | |||||
書誌情報 |
Neuropeptides 巻 45, 号 4, p. 263-272, 発行日 2011-08 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01434179 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00324063 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 21549427 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.npep.2011.04.001 | |||||
権利 | ||||||
権利情報 | © 2011 Elsevier Ltd. NOTICE: this is the author's version of a work that was accepted for publication in Neuropeptides. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Neuropeptides Vol.45 Issue 4, Pages:263-272. doi:10.1016/j.npep.2011.04.001 |
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著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/114622 | |||||
識別子タイプ | HDL |