WEKO3
アイテム
{"_buckets": {"deposit": "af74fe61-760c-4580-8327-3630bf04850a"}, "_deposit": {"id": "15327", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "15327"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00015327", "sets": ["516"]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "12", "bibliographicPageEnd": "2428", "bibliographicPageStart": "2419", "bibliographicVolumeNumber": "6", "bibliographic_titles": [{"bibliographic_title": "Eukaryotic cell"}]}]}, "item_5_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "森尾, 貴広"}], "nameIdentifiers": [{"nameIdentifier": "193295", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "10292509", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000010292509"}, {"nameIdentifier": "0000000035", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000000035"}]}, {"creatorNames": [{"creatorName": "桑山, 秀一"}], "nameIdentifiers": [{"nameIdentifier": "132", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "40397659", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000040397659"}, {"nameIdentifier": "0000001199", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001199"}]}]}, "item_5_description_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}, {"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Ammonia has been shown to function as a morphogen at multiple steps during development of\nthe cellular slime mold Dictyostelium discoideum; however, it is largely unknown how\nintracellular ammonia levels are controlled. In the Dictyostelium genome, there are five genes\nthat encode putative ammonium transporters, amtA, amtB, amtC, rhgA, and rhgB. Here, we\nshow that AmtA regulates ammonia homeostasis during growth and development. We found\nthat cells lacking amtA had increased levels of ammonia/ammonium, whereas their extracellular\nammonia/ammonium levels were highly decreased. These results suggest that AmtA mediates\nexcretion of ammonium. Supporting a role for AmtA in ammonia homeostasis, AmtA mRNA is\nexpressed throughout the life cycle, and its expression level increases during development.\nImportantly, AmtA-mediated ammonia homeostasis is critical for many developmental processes.\namtA- cells are more sensitive to NH4Cl in chemotaxis towards cAMP and in formation of\nmulticellular aggregates. Furthermore, even in the absence of exogenously added ammonia, we\nfound that amtA- cells produced many small fruiting bodies and viability and germination of\namtA- spores were dramatically compromised. Taken together, our data clearly demonstrate\nthat AmtA regulates ammonia homeostasis and plays important roles in multiple developmental\nprocesses in Dictyostelium.", "subitem_description_type": "Abstract"}]}, "item_5_identifier_34": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2241/98226"}]}, "item_5_publisher_27": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Society for Microbiology"}]}, "item_5_relation_10": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "17951519", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1128/EC.00204-07", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2007, American Society for Microbiology"}]}, "item_5_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1535-9778", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11642650", "subitem_source_identifier_type": "NCID"}]}, "item_5_subject_20": {"attribute_name": "NIIサブジェクト", "attribute_value_mlt": [{"subitem_subject": "生物学", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yoshino, Ryuji"}], "nameIdentifiers": [{"nameIdentifier": "54798", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Morio, Takahiro"}], "nameIdentifiers": [{"nameIdentifier": "54799", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada, Yoko"}], "nameIdentifiers": [{"nameIdentifier": "54800", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuwayama, Hidekazu"}], "nameIdentifiers": [{"nameIdentifier": "54801", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sameshima, Masazumi"}], "nameIdentifiers": [{"nameIdentifier": "54802", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanaka, Yoshimasa"}], "nameIdentifiers": [{"nameIdentifier": "54803", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sesaki, Hiromi"}], "nameIdentifiers": [{"nameIdentifier": "54804", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iijima, Miho"}], "nameIdentifiers": [{"nameIdentifier": "54805", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-12-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "EC_6-12-Fig.pdf", "filesize": [{"value": "1.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1200000.0, "url": {"label": "EC_6-12-Fig.pdf", "url": "https://tsukuba.repo.nii.ac.jp/record/15327/files/EC_6-12-Fig.pdf"}, "version_id": "cf5e1a6b-c424-48b6-98fc-79c0e8b1dbaa"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-12-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "EC_6-12.pdf", "filesize": [{"value": "128.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 128600.0, "url": {"label": "EC_6-12.pdf", "url": "https://tsukuba.repo.nii.ac.jp/record/15327/files/EC_6-12.pdf"}, "version_id": "b8aaafe6-446c-43f3-974d-0a57efd9ec44"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Regulation of Ammonia Homeostasis by the Ammonium Transporter AmtA in Dictyostelium discoideum", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Regulation of Ammonia Homeostasis by the Ammonium Transporter AmtA in Dictyostelium discoideum"}]}, "item_type_id": "5", "owner": "1", "path": ["517", "516", "390"], "permalink_uri": "http://hdl.handle.net/2241/98226", "pubdate": {"attribute_name": "公開日", "attribute_value": "2008-02-08"}, "publish_date": "2008-02-08", "publish_status": "0", "recid": "15327", "relation": {}, "relation_version_is_last": true, "title": ["Regulation of Ammonia Homeostasis by the Ammonium Transporter AmtA in Dictyostelium discoideum"], "weko_shared_id": 5}
Regulation of Ammonia Homeostasis by the Ammonium Transporter AmtA in Dictyostelium discoideum
http://hdl.handle.net/2241/98226
http://hdl.handle.net/2241/98226b823b89f-490a-464b-95de-e251c2372019
名前 / ファイル | ライセンス | アクション |
---|---|---|
EC_6-12-Fig.pdf (1.2 MB)
|
|
|
EC_6-12.pdf (128.6 kB)
|
|
Item type | Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2008-02-08 | |||||
タイトル | ||||||
タイトル | Regulation of Ammonia Homeostasis by the Ammonium Transporter AmtA in Dictyostelium discoideum | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Yoshino, Ryuji
× Yoshino, Ryuji× Morio, Takahiro× Yamada, Yoko× Kuwayama, Hidekazu× Sameshima, Masazumi× Tanaka, Yoshimasa× Sesaki, Hiromi× Iijima, Miho |
|||||
著者別名 |
森尾, 貴広
× 森尾, 貴広× 桑山, 秀一 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Ammonia has been shown to function as a morphogen at multiple steps during development of the cellular slime mold Dictyostelium discoideum; however, it is largely unknown how intracellular ammonia levels are controlled. In the Dictyostelium genome, there are five genes that encode putative ammonium transporters, amtA, amtB, amtC, rhgA, and rhgB. Here, we show that AmtA regulates ammonia homeostasis during growth and development. We found that cells lacking amtA had increased levels of ammonia/ammonium, whereas their extracellular ammonia/ammonium levels were highly decreased. These results suggest that AmtA mediates excretion of ammonium. Supporting a role for AmtA in ammonia homeostasis, AmtA mRNA is expressed throughout the life cycle, and its expression level increases during development. Importantly, AmtA-mediated ammonia homeostasis is critical for many developmental processes. amtA- cells are more sensitive to NH4Cl in chemotaxis towards cAMP and in formation of multicellular aggregates. Furthermore, even in the absence of exogenously added ammonia, we found that amtA- cells produced many small fruiting bodies and viability and germination of amtA- spores were dramatically compromised. Taken together, our data clearly demonstrate that AmtA regulates ammonia homeostasis and plays important roles in multiple developmental processes in Dictyostelium. |
|||||
書誌情報 |
Eukaryotic cell 巻 6, 号 12, p. 2419-2428, 発行日 2007-12 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1535-9778 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11642650 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 17951519 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1128/EC.00204-07 | |||||
権利 | ||||||
権利情報 | © 2007, American Society for Microbiology | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | American Society for Microbiology | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/98226 | |||||
識別子タイプ | HDL | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |